주메뉴영역

KAIST

뒤로

KAIST Annual R&D Report

Designer proteins with chemical modifications

Writer : KAIST Date : 2017-09-22 Hit : 2542 Email : jsoh33@kaist.ac.kr
Author : Hee Sung Park Publication Date : 2016
Summary:
We developed a new strategy for installating authentic posttranslational modifications into recombinant proteins. We first create a phosphoserine-containing protein using the established O-phosphoserine orthogonal translational system. The phosphoserine residue is then dephosphorylated to dehydroalanine. Finally, Zn-Cu promoted conjugate addition to dehydroalanine of alkyl iodides enables chemo-selective carbon-carbon bond formation. Using well-tailored alkyl iodide and further refinements, the current scheme can be extended to produce designer proteins with diverse forms of authentic posttranslational modifications.
Publication text:

Designer proteins with chemical modifications 이미지1